LI-COR Biosciences has released a new Technical Note for using E-PAGE 48- and 96-well gels the Odyssey Infrared Imaging System. The E-PAGE 48- and 96-well electrophoresis systems (Invitrogen Corporation, Carlsbad, CA) provide a high-throughput SDS-PAGE method for processing protein samples.
When used in conjunction with the iBlot(TM) Dry Blotting System, up to 96 different proteins samples can be electrophoresed and transferred to a membrane in as little as 40 minutes. Alternatively, a semi-dry transfer apparatus may be used for E-PAGE gel transfer, such as the Trans-Blot® Semi-Dry Electrophoretic Transfer Cell (Bio-Rad Laboratories, Hercules, CA).
Detection for blots processed in this manner can be performed using IRDye® Infrared Secondary Antibodies and the Odyssey Infrared Imaging System. IRDye antibody conjugates are available in two spectrally-distinct wavelengths; therefore, multiplex detection can be accomplished, increasing the number of analytes to as many as 192; alternatively, one of the wavelengths can be used for normalization to improve quantification accuracy. Altogether, this system provides a rapid, convenient, quantitative, and highly sensitive means for analyzing proteins.
The Odyssey Infrared Imaging System is uniquely equipped with two infrared channels for direct fluorescence detection on membranes with sensitivity that is equal to or better than chemiluminescence. The superior signal-to-noise ratio produced using near infrared dyes produces results not possible with traditional fluorescence-based imaging systems. LI-COR infrared systems are noted for providing superior imaging for a growing list of applications including Western blots, In-Cell Western assays, EMSA's, gel documentation and in vivo imaging.